SubtiBank SubtiBank
cotG [2020-06-29 10:00:13]
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cotG [2020-06-29 10:00:13]

outer spore coat protein
Locus
BSU_36070
Isoelectric point
10.67
Molecular weight
23.81 kDa
Protein length
195 aa Sequence Blast
Gene length
588 bp Sequence Blast
Function
resistance of the spore
Product
spore coat protein
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class V
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

    • Gene

    Coordinates
    3,717,238 3,717,825

    The protein

    Catalyzed reaction/ biological activity

  • required for the maturation of CotB PubMed

    • Modification

  • phosphorylated on Ser-15, Ser-39, and Thr-147 by CotH PubMed

    • Localization

  • outer spore coat, localization depends on CotE PubMed

    • Expression and Regulation

    Operons

    Genes
    cotG
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigK: sigma factor, PubMed, in SigK regulon

    • Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon
  • GerR: activation, PubMed, in GerR regulon

    • Regulation

  • expressed late during sporulation in the mother cell (SigE, SigK, GerE) PubMed

    • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • BKE36070 (cotG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTGAAATCCTCCTTTT, downstream forward: _UP4_TAATCTAAGTTTTCATTGTT
  • BKK36070 (cotG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTGAAATCCTCCTTTT, downstream forward: _UP4_TAATCTAAGTTTTCATTGTT

    • References

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